SLAS2016 Short Courses
Derivation of iPS Cells and Maintenance Techniques of iPS-Derived Cells for Use in High-Throughput Screening and Disease Modeling
Course Description
This discussion based course will provide general overview on the basic stem cells biology and laboratory techniques used to derive and maintain human induced pluripotent stem cell (iPS). The application of using iPS- derived lines in high-throughput screening (HTS) and disease modeling. Additional topics will include manual and semi-automated reprogramming techniques, characterization of iPS assays and Embryonic Stem cells (ES) protocols and HTS strategy with emphasis on the derivation of neural and other lineage cell lines.Who Should Attend:
- Individuals who desire to understand the technical requirements for deriving, maintaining, characterizing and differentiating human IPS dell lines
- Scientists and automation specialists with some tissue culture experience but little to no experience in working with ES or iPS cell lines.
- Those interested in developing ips / ips derived screening campaigns
How You Will Benefit From This Course:
- Gain an understanding of stem cell biology
- Learn how to derive and maintain iPS lines from human dermal fibroblasts
- Learn appropriate iPS/ES characterization assays with emphasis on FACS and FCM
- Learn how to conduct lineage specific differentiation protocols to produce physiologically relevant cell lines for disease modeling
- Direct contact with experienced stem cell scientists
Course Topics:
- Introduction to Stem Cell Biology
- Derivation and Maintenance of Stem cell lines
- Characterization Techniques
- Lineage Specific Differentiation Protocols
- Optimizing Screening Campaigns
Instructor Name(s)/Affiliation(s):
David J Kahler — NYU Langone Medical Center
Justin Ichida — University of Southern California
Instructor Bios:
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David J Kahler David Kahler earned his Ph.D. in Molecular Medicine and Immunology from the Medical College of Georgia in the lab of Andrew Mellor, where he studied the phenotype, function, and lineage of a rare subset of immunomodulatory dendritic cells found in humans and mice. While at the New York Stem Cell Foundation, Dr. Kahler served as the Director of the Flow Cytometry Core and Drug Discovery laboratories, and as Director of Laboratory Automation where he developed FCM/FACS based and automated technologies for the derivation, characterization and maintenance of human IPSC lines. Currently he is providing experimental design, assay development and data analysis support to clients of the RNAi HTS Core Laboratory at the NYU Langone School of Medicine. |
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Justin Ichida Justin Ichida completed his Ph.D. at Harvard Medical School in the laboratory of Dr. Jack Szostak and completed postdoctoral training in the laboratory of Dr. Kevin Eggan in the Department of Stem Cell and Regenerative Biology at Harvard University. Dr. Ichida was a New York Stem Cell Foundation Druckenmiller Fellow and a recipient of the NIH K99/R00 Pathway to Independence award from the National Institute of Neurological Disorders and Stroke. Currently, Justin is an Assistant Professor of Stem Cell Biology and Regenerative Medicine at the University of Southern California. His current research uses patient-specific disease models to define the mechanisms that lead to neuronal loss in ALS and frontotemporal dementia. |
